| KRAS Mutation |
Sample ID |
Mutation (%) |
Lowest DNA Input with Correct Call
(ng/PCR)a |
| G12A, GGT > GCT |
FFPET #10, DNA blend |
4.5 |
12.5 |
| FFPET #11, DNA blend |
5.1 |
12.5 |
| G12D, GGT > GAT |
FFPET #12, DNA blend |
5.0 |
12.5 |
| FFPET #13, DNA blend |
5.2 |
12.5 |
| G12S, GGT >AGT |
FFPET #14, DNA blend |
5.6 |
12.5 |
| G12V, GGT > GTT |
FFPET #15, DNA blend |
2.9 |
12.5 |
| FFPET #16, DNA blend |
5.7 |
12.5 |
| G13A, GGC > GCC |
Plasmid, DNA blend |
3.7 |
50.0b |
| G13D, GGC > GAC |
FFPET #17, DNA blend |
4.8 |
3.1 |
| FFPET #18, DNA blend |
6.8 |
3.1 |
| G13S, GGC >AGC |
Plasmid, DNA blend |
1.6 |
50.0b |
| G13V, GGC > GTC |
Plasmid, DNA blend |
2.6 |
50.0b |
| Q61E, CAA >GAA |
Plasmid, DNA blend |
5.7 |
50.0b |
| Q61P, CAA > CCA |
Plasmid, DNA blend |
3.2 |
50.0b |
| Q61R, CAA > CGA |
FFPET #19, DNA blend |
4.8 |
50 |
| aLowest amount of input DNA (ng/PCR) tested with a correct mutation call rate of ≥95% (upper one-sided 95% CI) per 24 replicates.
bPlasmid DNA blends were tested only at 50.0 ng per PCR reaction. |
|
