Sample Sanger Sequencing Results cobasKRAS
Test Results
454 Sequencing Results (% Mutation) Concordance After
454 Resolution
Lot 1 Lot 2 cobas
KRAS Test
Lot 1
cobas
KRAS Test
Lot 2
1 codon 12 NMD NMD NMD (<1%) Yes Yes
2 codon 12 NMD NMD NMD (<1%) Yes Yes
3 codon 12 NMD NMD NMD (<1%) Yes Yes
4 codon 12 NMD NMD NMD (<1%) Yes Yes
5 codon 12 NMD NMD NMD (<1%) Yes Yes
6 codons 12 & 61 codon 12/13 codon 12/13 codon 12 (23.7%) Yes Yes
7 NMD codon 12/13 codon 12/13 codon 12 (59.2%) Yes Yes
8 NMD codon 12/13 codon 12/13 codon 12 (13.3%) Yes Yes
9 NMD codon 12/13 codon 12/13 codon 12 (4.9%) Yes Yes
10 NMD codon 12/13 codon 12/13 codon 12 (9.7%) Yes Yes
11 NMD codon 12/13 codon 12/13 codon 12 (39.3%) Yes Yes
12 NMD codon 12/13 codon 12/13 codon 12 (3.0%) Yes Yes
13 NMD NMD codon 12/13 codon 12 (1.2%) No Yes
14 NMD NMD codon 12/13 codon 12 (3.7%) No Yes
15 NMD codon 61 codon 61 codon 59 (2.8%) No No
16 NMD NMD invalid codon 13 (1.8%) No N/A
NMD, no mutation detected
Table 4: Resolution of discordant results by 454 sequencing for method correlation