Sample Sanger Sequencing Results cobasKRAS Test Results 454 Sequencing Results (% Mutation) Concordance After 454 Resolution Lot 1 Lot 2 cobas KRAS Test Lot 1 cobas KRAS Test Lot 2 1 codon 12 NMD NMD NMD (<1%) Yes Yes 2 codon 12 NMD NMD NMD (<1%) Yes Yes 3 codon 12 NMD NMD NMD (<1%) Yes Yes 4 codon 12 NMD NMD NMD (<1%) Yes Yes 5 codon 12 NMD NMD NMD (<1%) Yes Yes 6 codons 12 & 61 codon 12/13 codon 12/13 codon 12 (23.7%) Yes Yes 7 NMD codon 12/13 codon 12/13 codon 12 (59.2%) Yes Yes 8 NMD codon 12/13 codon 12/13 codon 12 (13.3%) Yes Yes 9 NMD codon 12/13 codon 12/13 codon 12 (4.9%) Yes Yes 10 NMD codon 12/13 codon 12/13 codon 12 (9.7%) Yes Yes 11 NMD codon 12/13 codon 12/13 codon 12 (39.3%) Yes Yes 12 NMD codon 12/13 codon 12/13 codon 12 (3.0%) Yes Yes 13 NMD NMD codon 12/13 codon 12 (1.2%) No Yes 14 NMD NMD codon 12/13 codon 12 (3.7%) No Yes 15 NMD codon 61 codon 61 codon 59 (2.8%) No No 16 NMD NMD invalid codon 13 (1.8%) No N/A NMD, no mutation detected

Table 4: Resolution of discordant results by 454 sequencing for method correlation