Figure 2: Diagram describing the SNaPshot primer location on the HIV-1genome [HXB2, K03455]. The slanted line at the 5' end of the primers indicatenon specific extension bases which is a 15 base poly A stretch of M41L probeand 44 base 'GATC' repeat, occurring 11 times in M184V probe. The locationof target mutation M41L and M184V are shone within black stars and arepositioned immediately adjacent to the last base at the 3' end of each probes.The hybridization location of M41L and M184V probes are on different strands ofthe HIV-1 genome and hence designed to extend in opposite directions.