Gram negative Bacteria DCM(mm) n-butanol(mm) Ethyl acetate (mm) Methanol (mm) Standard (mm) Control Vibrio parahemolyticus 7 ± 0 7 ± 0 0 ± 0 7 ± 0.5 26.5 ± 3.5 0 ± 0 Vibrio mimicus 7 ± 0 7.5 ± 0.5 7.5 ± 0.05 7.5 ± 0.5 25.5 ± 0.5 0 ± 0 Salmonella paratyphi 0 ± 0 7.5 ± 0.5 0 ± 0 7.5 ± 0.5 28.5 ± 0.5 0 ± 0 Salmonella typhi 0 ± 0 7 ± 0 12 ± 0.1 8 ± 0 30 ± 0 0 ± 0 Shigelladysenteriae 0 ± 0 0 ± 0 0 ± 0 7 ± 0 29 ± 0 0 ± 0

Table 3: Anti-bacterial activity of the methanolic extract of Aegle marmelos (fruit), VLC fraction of Dicloro methen, n-butanol, Ethyl acetate, methanol against gram negative bacteria.