Graph 3: Relative migration efficacy for the different sorts of above mentioned cell lines. (The first bar is for the positive control (chemokine RANTES), with that the migration of macrophages had been recorded on lower membrane of the migration chamber. The second bar is for the B16F10 mouse mellnoma cell lines, the migrated mecrophases were of 100% as relative the others. The third bar is for co culture B16F10 mouse melanoma cells and α-RANTES antibody, the relative migration of the macrophages were deceased to about 56 %. The relative migration of macrophages towards the co culture B16F10 mouse melanoma cell line and CCR2 antagonist on the lower membrane of chamber was 60 %).