Solution 12% resolving gel 4% stacking gel Acrylamide 590 μl 330 μl 1.5 M tris/HCl (pH 8.8) 650 μl - 0.5 M tris/HCl (pH 6.6) - 650 μl ddH2O 1.24 ml 1.51 ml SDS 13 μl 13 μl TEMED 3 μl 3 μl APS 13 μl 13 μl

Table 6: Componets for the preparation of resolving and stacking gel.