Time post single dosing   6 hrs     24 hrs   72  hrs 168  hrs
Dose (mg/kg) 0 100 1250 0 100 1250 0 1250 0 1250
Number of animals 4 5 5 4 5 7 4 7 4 7
Histopathology (Liver)                    
Necrosis, centrilobular - - - - - + (1/7) ++ (1/7) +++ (2/7) - + (3/7) +++ (1/7) - -
Fibrosis, centrilobular - - - - - - - + (3/7) ++ (1/7) - + (2/7)
Mitosis, centrilobular - - - - - - - + (2/7) - -
Hepatocyte glycogen depletion - - - - - + (3/7) ++ (2/7) ++++ (2/7) - - - -
Blood chemistry                    
ALT (U/L) 32.5±4.0 36.4 ± 4.0 36.8 ± 1.0 67.5 ± 3.0 55.4 ± 5.0** 459.9 ± 673.5 53.8 ± 8.5 57.4 ± 5.4 58.0 ± 5.1 59.0 ± 8.7
AST (U/L) 110.3±17.0 106.2 ± 24.9 92.8 ± 8.4 98.1 ± 14.1 102.8 ± 19.5 3007 ± 5166 87.5 ± 9.3 106.4 ± 34.7 106.8 ± 40.4 103.3 ± 22.8
ALP (U/L) 195.3 ± 42.6 211.4 ± 77.7 184.3+24.2 353.0+89.2 309.6+47.5 365.4+63.2 285.8+30.1 305.4+63.7 319.0+44.3 306.4 ± 64.3
GGT (U/L) 5.5 ± 0.6 5.6 ± 1.7 6.5 ± 0.6* 7.3 ± 1.3 6.4 ± 1.1 6.4 ± 1.5 6.8 ± 1.0 7.7 ± 2.1 6.3 ± 1.3 7.0 ± 1.2
TBILI (mg/dL) 0.40 ± 0.40 0.28 ± 0.15 0.20 ± 0.08 0.28 ± 0.05 0.32 ± 0.08 0.38 ± 0.16 0.20 ± 0.11 0.28 ± 0.17 0.25 ± 0.10 0.18 ± 0.07
BUN (mg/dL) 17.0 ± 1.4 16.6 ± 1.8 11.5 ± 1.7** 10.0 ± 0.8 12.8 ± 3.1 12.3 ± 4.7 11.8 ± 1.7 16.6 ± 7.0 16.0 ± 1.8 11.4 ± 4.0
CRE (mg/dL) 0.38 ± 0.13 0.36 ± 0.05 0.35 ± 0.06 0.38 ± 0.10 0.38 ± 0.08 0.40 ± 0.12 0.43 ± 0.05 0.48 ± 0.18 0.43 ± 0.10 0.38 ± 0.04
CHOL (mg/dL) 63.8 ± 8.7 61.4 ± 13.6 78.5 ± 15.5 61.3 ± 10.8 60.6 ± 5.4 92.0 ± 19.6* 77.3 ± 11.4 80.6 ± 11.1 69.5 ± 6.6 67.7 ± 10.6
TRI (mg/dL) 70.5 ± 50.2 77.8 ± 24.2 98.5 ± 30.2 170.0 ± 38.7 156.2 ± 56.7 179.6 ± 106.0 74.3 ± 20.0 96.9 ± 51.9 115.0 ± 59.1 101.6 ± 23.6
GLU (mg/dL) 251.5 ± 79.5 186.6 ± 15.9 165.8 ± 21.8 171.5 ± 15.1 175.2 ± 18.4 164.3 ± 26.5 193.0 ± 39.5 170.3 ± 4.8 186.0 ± 26.7 170.4 ± 13.2
Values for blood chemistry represented as mean + SD. * and ** represent p<0.05 and p<0.01, respectively. Histopathological lesions were qualified as:+, minimal; ++, mild; +++, moderate; ++++, marked
Table 1: Blood chemistry and histopathology.