metabolites retention time P valuea A(normal) B(cancerb) Rc
lactic acid 7.787 0.001 0.0278 ± 0.0038 0.0369 ± 0.0059 0.33
glycerol 13.253 0.001 0.0864 ± 0.0251 0.0358 ± 0.0158 -0.59
butanedioic acid 14.701 0.005 0.0191 ± 0.0035 0.0138 ± 0.0028 -0.28
L-serine 16.014 0.002 0.0009 ± 0.0001 0.0006 ± 0.0002 -0.33
L-threonine 16.832 0.008 0.0016 ± 0.0002 0.0010 ± 0.0008 -0.38
malic acid 20.084 0.033 0.0069 ± 0.0019 0.0046 ± 0.0020 -0.33
citric acid 29.589 0.003 0.1833 ± 0.0343 0.1166 ± 0.0478 -0.36
hexadecanoic acid 35.467 0.003 0.1402 ± 0.0202 0.1877 ± 0.0376 0.34
aP values were calculated based on Mann-Whitney test (significance at P<0.05). bCancer group includes the non-metastasis group and the metastasis group. cR value was calculated from the arithmetic mean values of each group. R=(B-A)/A. R with a positive value indicates a relatively higher concentration present in cancer group while a negative value means a relatively lower concentration as compared to the normal.
Table 2a: List of different metabolites identified in normal control group and cancer group (metastasis and non-metastasis mice).