metabolites

retention time

P valuea

A(normal)

B(cancerb)

Rc

lactic acid

7.787

0.001

0.0278±0.0038

0.0369±0.0059

0.33

glycerol

13.253

0.001

0.0864±0.0251

0.0358±0.0158

-0.59

butanedioic acid

14.701

0.005

0.0191±0.0035

0.0138±0.0028

-0.28

L-serine

16.014

0.002

0.0009±0.0001

0.0006±0.0002

-0.33

L-threonine

16.832

0.008

0.0016±0.0002

0.0010±0.0008

-0.38

malic acid

20.084

0.033

0.0069±0.0019

0.0046±0.0020

-0.33

citric acid

29.589

0.003

0.1833±0.0343

0.1166±0.0478

-0.36

hexadecanoic acid

35.467

0.003

0.1402±0.0202

0.1877±0.0376

0.34

aP values were calculated based on Mann-Whitney test (significance at P<0.05). bCancer group includes the non-metastasis group and the metastasis group. cR value was calculated from the arithmetic mean values of each group. R=(B-A)/A. R with a positive value indicates a relatively higher concentration present in cancer group while a negative value means a relatively lower concentration as compared to the normal.
Table 2a: List of different metabolites identified in normal control group and cancer group (metastasis and non-metastasis mice).