metabolites retention time P valuea A(non-metastasis) B(metastasis) Rb
glycerol 13.253 0.046 0.0437 ± 0.0157 0.0278 ± 0.0119 -0.36
butanedioic acid 14.701 0.046 0.0153 ± 0.0025 0.0124 ± 0.0024 -0.19
L-serine 16.014 0.006 0.0007 ± 0.0001 0.0005 ± 0.0002 -0.29
L-threonine 16.832 0.027 0.0014 ± 0.0008 0.0005 ± 0.0005 -0.64
malic acid 20.084 0.036 0.0055 ± 0.0018 0.0036 ± 0.0019 -0.35
L-proline 21.329 0.046 0.0101 ± 0.0040 0.0064 ± 0.0024 -0.37
myo-inositol 36.316 0.003 0.0062 ± 0.0013 0.0103 ± 0.0027 0.66
aP values were calculated based on Mann-Whitney test (significance at P<0.05). bR value was calculated from the arithmetic mean values of each group. R=(B-A)/A. R with a positive value indicates a relatively higher concentration present in metastasis group while a negative value means a relatively lower concentration as compared to the non-metastasis.
Table 2b: List of different metabolites identified in metastasis and non-metastasis groups.