metabolites

retention time

P valuea

A (non-metastasis)

B (metastasis)

Rb

glycerol

13.253

0.046

0.0437±0.0157

0.0278±0.0119

-0.36

butanedioic acid

14.701

0.046

0.0153±0.0025

0.0124±0.0024

-0.19

L-serine

16.014

0.006

0.0007±0.0001

0.0005±0.0002

-0.29

L-threonine

16.832

0.027

0.0014±0.0008

0.0005±0.0005

-0.64

malic acid

20.084

0.036

0.0055±0.0018

0.0036±0.0019

-0.35

L-proline

21.329

0.046

0.0101±0.0040

0.0064±0.0024

-0.37

myo-inositol

36.316

0.003

0.0062±0.0013

0.0103±0.0027

0.66

aP values were calculated based on Mann-Whitney test (significance at P<0.05). bR value was calculated from the arithmetic mean values of each group. R=(B-A)/A. R with a positive value indicates a relatively higher concentration present in metastasis group while a negative value means a relatively lower concentration as compared to the non-metastasis.
Table 2b: List of different metabolites identified in metastasis and non-metastasis groups.