Protein Name

Stage 1

Stage 2

Stage 3

Stage4

N

T

N

T

N

T

N

T

Aldolase B

53

ND

45

ND

85

ND

79

39

Gamma Enolase

ND

63

ND

12

ND

46

ND

29

Adipophilin

ND

63

ND

12

ND

46

ND

29

TGF-beta induced

23

107

14

12

12

36

11

20

Vimentin

107

168

60

62

96

189

201

271

Isocitrate DH

11

3

11

2

11

ND

8

3

Gelsolin

11

13

12

2

8

7

8

12

Ceruloplasmin

9

10

9

6

3

8

12

16

Table 1: Selected expression of putative ccRCC protein biomarkers in pooled normal (N) and tumor (T) tissues representative of Stage 1-4 cancers. Individual renal tissues (10-15 mg) from matched tumor and normal pairs were processed by trifluoroethanol (TFE) solubilization and sonication as adapted from Wang et al. [104]. Following trypsin digestion, the resulting peptides were separated by MudPIT and analyzed for protein identification as described in the legend to Table 2. Results are listed as spectral counts for each indicated protein.