| Gene |
TaqMan probe No. |
forward primer 5’ → 3’ |
Reverse primer 5’ → 3’ |
| CGB* |
#71 (Cat. No. 04688945001) |
TACTGCCCCACCATGACC |
CACGGCGTAGGAGACCAC |
| GNRH1 |
#29 (Cat. No. 04687612001) |
GACCTGAAAGGAGCTCTGGA |
CTTCTGGCCCAATGGATTTA |
| MET |
#67 (Cat. No. 04688660001) |
CGTCAACAAAAACAATGTGAGAT |
AAACTCTGTTCGATATTCATCACG |
| KRT19 |
#71 (Cat. No. 04688945001) |
GCCACTACTACACGACCATCC |
CAAACTTGGTTCGGAAGTCAT |
| HPRT |
Human HPRT Gene Assay, Cat. No. 05 046 157 001 (Roche Diagnostics) |
| *The nucleotide sequences of primers were designed to enable amplification of all genes encoding beta subunit of human chorionic gonadotropin (CGB1-9) |
