Figure 1: Gene expression (Mean Fluorescence Intensity) using cell line derived RNA and Formalin Fixed Paraffin Embedded (FFPE) derived RNA. (A) Inter-run correlation of gene expression using purified RNA derived from a breast cancer cell line MDA-MB-453. (B) Samples were microdissected and the same sample was analysed following H&E staining (y-axis) or unstained (x-axis). The relative expression levels did not differ and hence the assay can be used on stained microdissected FFPE sample lysates.