Figure 6: Effects of resveratrol on hepatic hypoxia inducible factor (HIF)-1a expression during DENA-initiated hepatocarcinogenesis in female Sprague-Dawley rats. Rats were sacrifi ced 20 weeks following the commencement of the study and immunohistochemistry was performed to detect HIF-1a. Arrowheads indicate immunohistochemical staining of HIF-1a. (A) Near absence of immunopositivity in normal liver (100×); (B) and (C) intense immunoreactivity in DENA control liver (100 and 250× respectively); (D) no alteration of expression in 50 mg/kg group (100×); (E) decreased expression in 100 mg/kg resveratrol group (100×); and (F) near absence of HIF-1a expression in 300 mg/kg resveratrol group (100×) are noticed.