GO-term

Function

0006917

Induction of apoptosis

0043068

Positive regulation of programmed cell death

0008633

Activation of pro-apoptotic gene products

0043281

Regulation of caspase activity

0008632

Apoptotic program

0007160

Cell-matrix adhesion

Table 2: Affymetrix protocol. The data generated from the Affymetrix® microarray analysis revealed that the differentiated, bone-matrix-producing hFOB1.19 osteoblasts affected the gene expression in PC-3 cells substantially more than the osteprogenitor-like, undifferentiated osteoblasts did. Interestingly, filtered GO-data showed showed specific gene classes which was affected by the co culture between PC-3 cells and differentiated osteoblasts. The genes belonging to the gene class are predominantly repressed by co-culturing, and the mainly classes positive regulate apoptosis and cell adhesion.