Figure 3:Expression levels of several leptin-targeted molecules in DMBA-induced MT and mammary tissues from controls and PEG-LPrA2 treated mice. (A) Western blot (WB) analysis of leptin-related molecules, (B) quantitative analysis of protein levels for Notch (receptors, ligands and target genes) in malignant and tumor-free mammary tissues. Quantitative WB data were calculated from densitometric analysis of bands with the NIH image program. The values were normalized to β-actin as a control. (a) p<0.05 when comparing levels of protein in mammary glands from DIO-mice untreated (DIO-C) to DIO-mice treated with PEG-LPrA. (b) p<0.05 when comparing levels of protein in MT to those in mammary glands from DIO-mice untreated (DIO-C) or treated with PEG-LPrA. Data (mean ± standard error) representative results derived from a minimum of 3 independent experiments.