Figure 4: Plasma and tissue levels of leptin and leptin-related cytokines in mice challenged with DMBA. Lean (n=20; fed low-fat diet: 10% cal-fat) and DIO-mice (n=28; fed high-fat diet, HFD: 55% cal-fat) were treated with PEG-LPrA2 [once (I) or twice (II) a week] or received saline injections for 32 weeks. Levels of leptin (ng/ml); VEGF (ng/ml); adiponectin (ng/ml x 103); and IL-1α and β (pg/ml x 10) in plasma (A and C) from lean and DIO-mice, respectively, as determined by ELISA. Levels of leptin (ng/ml/mg-protein); VEGF (ng/ml/mg-protein); and IL-1α and β (pg/ml/mg-protein x 10) in mammary and MT tissue homogenates (B and D) from lean and DIO-mice, respectively, as determined by ELISA. (a) p<0.05 when comparing levels to lean (L-C) and obese controls (DIO-C) without MT. Data (mean ± standard error) representative results derived from triplicate determinations.