Figure 2: Adult flies expressing dRFX3 in wing imaginal disc exhibited atrophied wing and split thorax phenotypes. (a)~(c) Micrographs of adult flies. (d)~(f) Immunostainning of wing imaginal discs with anti-HA antibody. (A) (a, d) sd-GAL4/+; +/+, (b, e) sd-GAL4/+; UAS-HA-dRFX3 (strain 18) /+, (c, f) sd-GAL4/+; UAS-HA-d RFX3 (strain 53) /+, (B) (a, d) +/+; pnr-GAL4/+, (b, e) +/+; pnr-GAL4/UAS-HA-dRFX3 (strain 18) /+, (c, f) +/+; pnr-GAL4/UAS-HA-dRFX3 (strain 53) /+. These flies were developed at 28oC. HA signals are detected in ventral regions (A-e and A-f) or dorsal regions (B-e and B-f) of wing discs. Arrows indicate the atrophied wing. Red-circled regions indicate the split thorax of the adult fly.