Figure 4: Overexpression of HA-dRFX3 in eye imaginal disc exerted no effect on the cell cycle progression. (A) After incorporation of BrdU, the eye imaginal discs were immuno-stained with anti-BrdU antibody. (a) GMR-GAL4/+; +/+, (b) GMR-GAL4/+; UAS-HA-dRFX3 (strain 18) /+, (c) GMR-GAL4/+; UASHA- dRFX3 (strain 53) /+. (B) Immunostaining of eye discs with anti-Cyclin B antibody. (a) GMR-GAL4/+; +/+, (b) GMR-GAL4/+; UAS-HA-dRFX3 (strain 18)/+, (c) GMR-GAL4/+; UAS-HA-dRFX3 (strain 53) /+. Arrows indicate morphogenetic furrow (MF). Blue arrowheads indicate BrdU-positive cells behind MF and white arrowheads indicate Cyclin B-positive cells behind MF.