Figure 5: Overexpression of dRFX3 in the eye imaginal discs interfered with the differentiation of R2/R5, R1/R6 and R7 differentiation, but exerted no effect on that of R8. Males of the strain X63 were crossed with females of GMR-GAL4; + or GMR-GAL4; UAS-HA-dRFX3. (A) GMR-GAL4/+; +; X63/+, (B) GMR-GAL4/+; UAS-HA-dRFX3 (strain 18) /+; X63/+, (C) GMRGAL4/+; UAS-HA-dRFX3 (strain 53) /+; X63/+. The X63 expresses lacZ in R8 and R2/R5. Males of the strain AE127 were crossed with females of GMRGAL4; + or GMR-GAL4; UAS-HA-dRFX3. (D) GMR-GAL4/+; +; AE127/+, (E) GMR-GAL4/+; UAS-HA-dRFX3 (strain 18) /+; AE127/+, (F) GMR-GAL4/+; UAS-HA-dRFX3 (strain 53) /+; AE127/+. The AE127 expresses lacZ in R3/ R4 and R1/R6. Males of the strain p82 were crossed with females of GMRGAL4; + or GMR-GAL4; UAS-HA-dRFX3. (G) GMR-GAL4/+; +; p82/+, (H) GMR-GAL4/+; UAS-HA-dRFX3 (strain 18) /+; p82/+, (I) GMR-GAL4/+; UASHA- dRFX3 (strain 53) /+; p82/+. The p82 expresses lacZ in R3/R4 and R7. The eye imaginal discs of these progenies were immunostained with antib- galactosidase antibody.