P53 gene positions Primer Sequence Tm PCR Product size
Exon 4 (Codon 72) Arg>Pro27 Arginine allele
A1: 5'-TCCCCCTTGCCGTCCCAA-3'
A2: 5'-CTGGTGCAGGGGCCACGC-3'
60°C 141bp
Proline allele
P1:5'-GCCAGAGGCTGCTCCCCC-3'
P2:5'-CGTGCAAGTCACAGACTT-3'
55°C 177bp
Exon 5 (Codon168) Cytosine deletion 28 F1(wt) 5'-CTACAAGCAGTCACAGCAC-3'
F2(mt)5'-CTACAAGCAGTCACAGCAA-3'
R (c) ATCAGTGAGGAATCAGAGG-3'
55°C 150bp
Exon 8(Codon 282) C>T (Arg>Trp) 28 F1(wt) 5'-CTTGTCCTGGGAGAGACC-3'
F2(mt) 5'-CTTGTCCTGGGAGAGACT-3'
R(c) 5'-GAGCTGGTGTTGTTGGGC-3'
58°C 205bp
Table 2: Primers used for p53 polymorphism and mutations.