P53 gene positions Primer Sequence Tm PCR Product size
Exon 4 (Codon 72) Arg>Pro27 Arginine allele A1: 5'-TCCCCCTTGCCGTCCCAA-3' A2: 5'-CTGGTGCAGGGGCCACGC-3' 60°C 141bp
Proline allele P1:5'-GCCAGAGGCTGCTCCCCC-3' P2:5'-CGTGCAAGTCACAGACTT-3' 55°C 177bp
Exon 5 (Codon168) Cytosine deletion 28 F1(wt) 5'-CTACAAGCAGTCACAGCAC-3' F2(mt)5'-CTACAAGCAGTCACAGCAA-3' R (c) ATCAGTGAGGAATCAGAGG-3' 55°C 150bp
Exon 8(Codon 282) C>T (Arg>Trp) 28 F1(wt) 5'-CTTGTCCTGGGAGAGACC-3' F2(mt) 5'-CTTGTCCTGGGAGAGACT-3' R(c) 5'-GAGCTGGTGTTGTTGGGC-3' 58°C 205bp
Table 2: Primers used for p53 polymorphism and mutations.