Figure 1: Particulate Cr(VI) Disrupts Cdc20 Localization to Kinetochores. This figure shows that chronic exposure to zinc chromate induces a concentration- and time-dependent decrease in Cdc20 localization to the kinetochores. A) The representative images of Cdc20 localization to the kinetochores after exposure to 0.2 ug/cm2 zinc chromate for 24-120 h. Blue (DAPI) represents the DNA, green (Alexa 488) represents the kinetochores (anti-centromere serum) and red (Alexa 555) represents Cdc20. B) This figure shows that 24 h exposure to 0-0.2 ug/cm2 zinc chromate induced no changes in Cdc20 kinetochore localization while more chronic exposures (48-120 h) decreased Cdc20 kinetochore localization. Data represent an average of three independent experiments ± the standard error of the mean. *Cells with no Cdc20 localization were statistically different from control (p<0.05), †Cells with partial Cdc20 localization were statistically different from control (p<0.05), ‡Cells with complete Cdc20 localization were statistically different from control (p<0.05).