Figure 3: Particulate Cr(VI) Decreases Phosphorylated Cdc20 Protein Levels. This figure shows that zinc chromate decreases phosphorylated Cdc20 protein levels after 24-120 h exposure. Immunoprecipitation was carried out from whole cell lysates with anti-Cdc20 beads and western blotting was performed with anti-phosphoserine antibody. A) Phosphorylated Cdc20 levels after a 24 or 120 h exposure to 0.2 ug/cm2 zinc chromate. Data represent an average of two experiments ± standard error of the mean. B) Representative western blot images of phosphorylated Cdc20 protein levels after total-Cdc20 immunoprecipitation.