Figure 5: Particulate Cr(VI) Alters Mad2 and Cdc20 Protein Interactions. This figure shows that zinc chromate alters Mad2-Cdc20 interactions after 24 or 120 h exposure. Co-immunoprecipitation was carried out from whole cell lysates with anti-Cdc20 beads and western blotting was performed with indicated antibodies. A) Mad2-phosphorylated Cdc20 interactions are decreased after both 24 and 120 h exposure with a dramatic impact at 120 h. B) Representative image of total-Cdc20, phosphorylated Cdc20 and total- Mad2 protein levels following Cdc20 immunoprecipitation after 24 and 120 h exposure. Data represent an average of two experiments ± standard error of the mean.