Figure 1: Acrolein inhibits N-terminal tail acetylation of newly synthesized histones. Cytosolic cell fractions, nuclear extracts, and soluble chromatin fractions were isolated from BEAS-2B or A549 cells treated with or without Acrolein (Acr) for 2 h and then subjected to Western blot. The results showed drastic decrease of cytosolic H4K12Ac and H3K9 and K14Ac by Acr exposure [91].