Figure 4: Inhibitory effects of HL on phosphorylation of Akt (Ser473) in A549 cells. (A) Cells were incubated with 300 μM HL for 3 or 24 hours. Wortmannin, an inhibitor of PI3K/Akt pathway, was used as a positive control. (B) Cells were incubated with 300-2500 μM HL for 3 hours. Cells were stained with Alexa Fluor 488 conjugated anti-phosho-Akt antibody and analyzed by flow cytometry.*Significantly different (p<0.05) compared with the controls (Student’s t-test); data are the mean ± standard error (n=3) from three independent experiments.