Dosage(μg/kg) Sample OD value   Number of antibody-positive animals
Week 4 Week 8 Week 4 Week 8
0 Serum 0.068 ± 0.006 0.068 ± 0.008 0/5 0/5
20 Serum 0.064 ± 0.004 0.073 ± 0.009 0/5 0/5
100 Serum 0.064 ± 0.007 0.073 ± 0.003 0/5 0/5
500 Serum 0.068 ± 0.019 0.072 ± 0.013 0/5 0/5
Positive antibody (anti-G-CSF) 1.109 ± 0.090  
aAdult Sprague-Dawley rats were injected daily with G-CSFa subcutaneously at the dose of 0 (vehicle control), 20, 100, or 500 μg/kg, for up to 4 weeks. Peripheral blood serum was obtained for ELISA analysis either at the end of the 4-week treatment period (week 4) or after a further 4-week recovery period (week 8)
Table 3: Incidence of binding antibody detection after repeated G-CSFa administration in ratsa.