Figure 4: Treatment with CDDO-Me alters methyl transferases and methylation status of hTERT promoter. A. Panc-1 and MiaPaCa-2 cells were untreated or treated with CDDO-Me (00- 0.5 μM) for 5 days and cellular levels of DNMT1 and DNMT3a DNA methyl transferases were measured by western blotting. B. Methylation status of CpGs at hTERT promoter 5’ transcription regulatory and intergenic transcription regulatory region (248 to +108 nucleotides) in Panc-1 cells was assessed by bisulfite sequencing as described in Materials and Methods. C. Data are presented as average percent change in methylated CpG sites in the hTERT regulatory region after treatment with CDDO-Me.