Figure 5: CDDO-Me induces chromatin modifications. A. Effect on transcriptionally active chromatin markers. Panc-1 and MiaPaCa-2 cells were treated with CDDO-Me (0-0.5 μM) for 5 days and cell lysates were analyzed for acetylated histone H3 (Lys 9), acetylated histone H4, dimethyl H3 (Lys 4) and tri-methyl H3 (Lys 9) by western blotting. B. CDDO-Me induces changes in histone modification in regulatory region of hTERT promoter. Panca-1 cells were treated with CDDO-Me as described above and active chromatin markers interacting with hTERT promoter (acetylated histone H3 (Lys 9), acetylated histone H4, di-methyl H3 (Lys 4) and tri-methyl H3 (Lys 9) were analyzed by ChIP-PCR assay as described in Materials and Methods. No antibody controls were included to verify ChIP efficacy. The experiment was repeated two times.