Figure 1: Effect of high fat diet feeding on parametrial fat pad size and crownlike structure density. A. Picture demonstrating parametrial fat pad (fat tissue) size after 4 months of LFD or HFD feeding. B. Parametrial fat was fixed in formalin and immunohistochemically stained with F4/80 for CLS. Pictures were taken under 100×microscopic magnification with a light microscope and are representative of data obtained from more than three mice. Parametrial fat from mice fed a HFD demonstrated larger adipocytes and a higher density of CLS (88.8 ± 8.0 mm2; dark brown stain) compared with mice fed a LFD (0.9 ± 0.2 mm2).