Locus Primers Accession number Size
(Gene Bank)
β-actin- F 5′-AGACGCAGGATGGCATGGG-3′  NM_001101.3  161bp
β-actin –R 5-GAGACCTTCAACACCCCAGCC-3′
COMT-F 5’-CTGGAGGCCATTGACACCTA -3′ NM_000754.3 202bp
COMT-R 5′-GGTTGATCTCGATGGTGATGAG -3′
Table 2: Primer sequences used in RT-PCR and Real time-PCR.