LANA
Figure 3: LANA co-localizes with p63 inside the nucleus. The nuclei are counterstained using DAPI (blue), Alexa Fluor 594 (red) is used for Flag-TAp63α and Alexa Fluor 488 (green) is used for Myc- LANA. The images were sequentially captured using confocal microscope and are merged, as required. Presence of both TAp63α (upper right panel) and LANA (upper left panel) in the nucleus is confirmed by comparing with DAPI staining (lower left panel). Colocalization of TAp63α and LANA are confirmed by the merged image (lower right panel) expressing a different color (yellow) along with the colors of Alexa Fluor 594 (red) and Alexa Fluor 488 (green). These results indicate an interaction between TAp63α and LANA and thus a role of LANA for modulating the activities of TAp63α, thereby helping the cells escape apoptosis.