protein
Figure 5: MEK and ERK protein expressions following siRNA knock down of Sos1 gene in Caco-2 cells. A) Western Blot showing the effect of siRNA treatment at different time intervals on MEK and ERK protein expressions. (B) Western Blot showing the effect of Sos1 siRNA treatment at different time intervals on active phospho- MEK and phospho-ERK protein expressions. CTRL represents the untreated control sample. β-Actin expression was utilized as a housekeeping gene for calibration.