Figure 7: MEK and ERK protein expressions following siRNA knock down of Sos1 gene in HT-29 cell line. Western Blot showing the effect of siRNA treatment at different time intervals in the HT-29 cells on the protein expression of MEK, phosphor MEK, ERK, and phosphor ERK. CTRL represents the untreated control sample. β-Actin expression was utilized as a housekeeping gene for calibration control.