| Primer |
Primer |
Product Size |
Working Tm (°C) |
| KRAS-Ex-1F |
5’CT CGGAGCTCGATTTTCCTA-3’ |
296 bp |
56.0 |
| KRAS-Ex-1R |
5’-GGGGACCCCTAATTCATTCA-3 |
| KRAS-Ex-2F |
5’-TTAACCTTATGTGTGACATGTTCTAA-3’ |
224 bp |
57.0 |
| KRAS-Ex-2R |
5’-AGAATGGTCCTGCACCAGTAA-3’ |
| KRAS-Ex-3F |
5’-TTCAAGTCCTTTGCCCATTT-3’ |
376 bp |
56.0 |
| KRAS-Ex-3R |
5’-TGCATGGCATTAGCAAAGAC-3’ |
| KRAS-Ex-4F |
5’-GATATTTGTGTTACTAATGACTGTGCT-3’ |
390 bp |
55.0 |
| KRAS-Ex-4R |
5’-AAGAAGCAATGCCCTCTCAA-3’ |
| BRAF-Ex-10F |
5’-AACATACTTGCTCCTCCTTAATGT-3’ |
261 bp |
58.0 |
| BRAF-Ex-10R |
5’-AAGGATAATATTACATTTGGCTGTGA-3’ |
| BRAF-Ex-11F |
5’- TTTCTTTTTCTGTTTGGCTTGA-3’ |
199 bp |
54.0 |
| BRAF-Ex-11R |
5’- ACTTGTCACAATGTCACCACA-3’ |
| BRAF-Ex-12F |
5’-TTAGTGAAAACACCCAAGAATGT- 3’ |
232 bp |
57.0 |
| BRAF-Ex-12R |
5’-CACTGGGAACCAGGAGCTAA- 3’ |
| BRAF-Ex-13F |
5’-GAATTGCTAAAGTTTGTCGACATTTT-3’ |
315 bp |
54.0 |
| BRAF-Ex-13R |
5’-TCCAAAAGAATAGCAGCCAAA-3’ |
| BRAF-Ex-15F |
5’-TGCTTGCTCTGATAGGAAAATG- 3’ |
227 bp |
55.0 |
| BRAF-Ex-15R |
5’-AGCATCTCAGGGCCAAAAAT- 3’ |
| BRAF-Ex-16F |
5’-AGGTTGTTTTTCAGTCTTTATTCAA-3’ |
258 bp |
55.0 |
| BRAF-Ex-16R |
5’-TTTTCTACAACTGGAGCCTTG-3’ |
| GAPDH-Ex-8-F |
5’-GGCTCTCCAGAACATCATCC-3’ |
240 bp |
56.0 |
| GAPDH-Ex-8-R |
5’-ACCTGGTGCTCAGTGTAGCC-5’ |
