Primer Primer Product Size Working Tm (°C)
KRAS-Ex-1F 5’CT CGGAGCTCGATTTTCCTA-3’ 296 bp 56.0
KRAS-Ex-1R 5’-GGGGACCCCTAATTCATTCA-3
KRAS-Ex-2F 5’-TTAACCTTATGTGTGACATGTTCTAA-3’ 224 bp 57.0
KRAS-Ex-2R 5’-AGAATGGTCCTGCACCAGTAA-3’
KRAS-Ex-3F 5’-TTCAAGTCCTTTGCCCATTT-3’ 376 bp 56.0
KRAS-Ex-3R 5’-TGCATGGCATTAGCAAAGAC-3’
KRAS-Ex-4F 5’-GATATTTGTGTTACTAATGACTGTGCT-3’ 390 bp 55.0
KRAS-Ex-4R 5’-AAGAAGCAATGCCCTCTCAA-3’
BRAF-Ex-10F 5’-AACATACTTGCTCCTCCTTAATGT-3’ 261 bp 58.0
BRAF-Ex-10R 5’-AAGGATAATATTACATTTGGCTGTGA-3’
BRAF-Ex-11F 5’- TTTCTTTTTCTGTTTGGCTTGA-3’ 199 bp 54.0
BRAF-Ex-11R 5’- ACTTGTCACAATGTCACCACA-3’
BRAF-Ex-12F 5’-TTAGTGAAAACACCCAAGAATGT- 3’ 232 bp 57.0
BRAF-Ex-12R 5’-CACTGGGAACCAGGAGCTAA- 3’
BRAF-Ex-13F 5’-GAATTGCTAAAGTTTGTCGACATTTT-3’ 315 bp 54.0
BRAF-Ex-13R 5’-TCCAAAAGAATAGCAGCCAAA-3’
BRAF-Ex-15F 5’-TGCTTGCTCTGATAGGAAAATG- 3’ 227 bp 55.0
BRAF-Ex-15R 5’-AGCATCTCAGGGCCAAAAAT- 3’
BRAF-Ex-16F 5’-AGGTTGTTTTTCAGTCTTTATTCAA-3’ 258 bp 55.0
BRAF-Ex-16R 5’-TTTTCTACAACTGGAGCCTTG-3’
GAPDH-Ex-8-F 5’-GGCTCTCCAGAACATCATCC-3’ 240 bp 56.0
GAPDH-Ex-8-R 5’-ACCTGGTGCTCAGTGTAGCC-5’
Table 1: Primers to amplify exons of the KRAS and BRAF genes.