Figure 2: The purity of CD8+ T cells after isolation. The purity of CD8+ T cell populations were determined before and after co-culture with HEp2 tumor transfectants and the levels of CD3+ T cells, CD8+ T cells and CD3-CD16+ NK cells were determined using surface staining with specific antibodies. The percentages of CD8+ T cells, NK cells and CD3+ T cells were determined by flow cytometric analysis of antibody stained cells. One of several representative experiments is shown in this figure.