Figure 4: Expansion of CD8+ T cell numbers after interaction with HEp2- IkB(S32AS36A) cells is in contrast with contraction of CD8+ T cells after interaction with vector-alone transfected HEp2 cells: (A) Purified CD8+ T cells (1X106 cells per sample) were treated with and without IL-2 (500u/ml) overnight before their co-culture with untreated and IFN-g pre-treated (200u/ ml), irradiated vector-alone and IκB(S32AS36A) transfected HEp2 cells (E:T ratio of 1:1). After two weekly stimulations with freshly supplemented IL2, the cells were removed from the CD8+ T /HEp2 cell co-cultures at day 18th and the numbers of effector cells were determined in each sample by microscopic counting of viable T cells by Trypan blue exclusion assay. The numbers of effector cells were determined by three independent observers. One of four representative experiments is shown in this figure. One of four representative experiments is shown in this figure.