Figure 5: Investigation of NPC exosome components altered by LMP1 expression. (A) Exosomes from NPC cells expressing LMP1 or vector control were separated by 1D SDS-PAGE and stained with coomassie blue. Protein bands were cut, digested with trypsin, and analyzed by MALDI MS/MS. (B) Equivalent amounts of total exosome protein in 2D lysis buffer were labeled with the indicated CyDye and separated by 2D gel-electrophoresis. Gels were scanned using a Typhoon scanner. Protein spots higher in LMP1 samples are green, those higher in vector control samples are red, and yellow spots1 represent proteins that are approximately at the same level between samples. (C) Proteins identified by MALDI MS/MS and Mascot database searches from gel shown in panel A. Proteins were considered to be significant with a total ion score greater than 66.