Figure 2: Treatment with PM induces apoptosis in prostate cancer cells. (A) LNCaP and PC-3 cells were treated with PM at 0 to 5 μM for 20 h. Cells were then reacted with 5 μl of annexin V-FITC and 5 μl PI for 30 min and percentage of annexin V-FITC binding cells was determined by flow cytometry. (B) Cleavage of PARP-1 in LNCaP and PC-3 cells treated with PM (0 to 10 μM) for 20 h was analyzed by western blotting. (C) Shows effect of PM (0 to 10 μM) on processing of procaspases-3 and -9 measured by western blotting. Similar results were obtained in three independent experiments.