RT-PCR MAGE-A1 Forward 5’-TGT GGG CAG GAG CTG GGC AA-3’
  MAGE-A1 Reverse 5’-GCC GAA GGA ACC TGA CCC AG-3’
  GAPDH Forward 5’-ATC TCT GCC CCC TCT GCT GA-3’
  GADPH Reverse 5’-GAT GAC CTT GCC CAC AGC CT-3’
MSP MAGE-A1_M Forward 5’-ATT TAG GTA GGA TTC GGT TTT C-3’
  MAGE-A1_M Reverse 5’-AAA CTA AAA CGT CTT CCC GCG-3’
  MAGE-A1_UM Forward 5’-ATT TAG GTA GGA TTT GGT TTT T-3’
  MAGE-A1_UM Reverse 5’-AAA CTA AAA CAT CTT CCC ACA-3’
Table 1: RT-PCR and MSP primer sequences of MAGE-A1 and GAPDH.