Figure 5: Chromatograms of nucleotide sequence analysis at and around codon 61. (A) The WT codon, CAA. (B) A mixture of CAA/ CTA codons shown by a minor peak of nucleotide “T” occurring under the major peak representing the second nucleotide of codon 61. (C) A mixture of CAA/ CGA codons shown by a minor peak “G” occurring under the major peak representing the second nucleotide of codon 61.