Figure 2: NOV-002 suppresses the phosphorylation of ErbB2 and PI3K subunit P85. (A) Immunoblots of phosphorylated ErbB2 and total ErbB2 in Colo205 and A549 cells with the treatment of various doses of NOV-002 or control (water). (B) Immunoblots of phosphorylated PI3K subunit P85 and total P85 in Colo205 and A549 cells with the treatment of various doses of NOV-002 or control (water). P85 phosphorylation was determined by immunoprecipitation using a P85 antibody, followed by immunoblot analysis with an antibody specific for phosphotyrosine.