Figure 1: Sensitization of SW480 cells and HCT8 cells to GTN/triciribine combination by H89. Exponentially growing (A) SW480 cells (3 × 105/mL) and (B) HCT8 cells (3 × 105/mL) were pretreated for 1 h 30 with an Akt specific inhibitor triciribine at indicated concentrations before exposure to GTN or/and H89 (10 μM each) for 24 hours or 48 hours. Apoptotic cells were counted after Hoechst 33342 staining. Results are the mean SD of 3 independent experiments. *p<0.05. (C) Western blot analysis of Akt1 and Akt2 isoforms in SW480 cells and HCT8 cells (1 representative of 3 independent experiments).