Figure 5: The mTORC2 activity inhibition is sufficient to enhance apoptosis induced by GTN/H89. (A) SW480 cells were transfected with rictor-specific siRNA or a control siRNA (mock) (20 nM each) for 48 hours. Total protein was isolated and subjected to western blot analysis for rictor expression. HSC70 was used as a control for equal protein loading (1 representative of 2 independent experiments). (B) Transfected cells were treated with GTN or/and H89 (10 μM each) for 24 hours to analyze by western blot Akt and pAkt ser 473 expression (1 representative of 2 independent experiments). (C) Transfected cells were treated with GTN or/and H89 (10 μM each) for 48 hours and apoptotic cells were counted after Hoechst 33342 staining (Results are the mean SD of 2 independent experiments).