Control (% relative to positive control) H89 ( % relative to positive control) Ratio H89/control ERk1 102 97 0,95 JNK1 9 2 0,24 JNK pan 2 9 3,87 P38g 12 3 0,25 P38d 1 0 0,00 RSK1 29 6 0,23 GSK- 3a/b 25 15 0,62 Akt-1 50 98 1,93 ERK-1 100 92 0.92 JNK- 2 6 0 0,00 P38-2 51 37 0,72 P38b 6 3 0,51 RSK2 0 0 0,00 GSK-3b 0 0 1.08 Akt 3 6 10 1,57 Akt pan 19 82 4,41 JNK 3 6 6 0,94 MSK 2 18 27 1,52 HSP 27 31 7 0,23 P 70 S6 kinase 3 0 0,06

Table 1: Phosphokinase profile of H89-stimulated SW480. Values represent the percentage of phosphorylated kinases relative to the positive control (% relative to pos. Control), an internal control supplied with the kit and represents 100% of the signal, in untreated SW480 cells (control) or treated with H89 (H89). The ratio (H89/control) represents the fold increase or decrease of phosphorylated kinases by H89.