Expression (mg/L) Cell Process Reference 1000 HEK 293* Fed-batch, high density transfection with plasmid cocktail, hypothermia and gene expression additives 67 300 CHO-S Batch transfection at moderate densities, optimized PEI/DNA/cell ratios, hypothermia 70 90 CHO DG44 Use of valproic acid, hypothermia, moderate transfection densities 71 80 CHO K1SV Fed-batch with DMSO and LiAC* 42 80 CHO-T Episomal replication system, batch transfection at low density 72 25 DG44 Low density transfection 73 22 DG44 Disposable orbital bioreactors 74 *HEK-293 cells are high producers, yet glycosylate unfavorably; DMSO (dimethyl sulfoxide); LiAC (Lithium Acetate)

Table 3: Antibody products produced with PEI-mediated TGE processes in mammalian cells.