Figure 5: hsa-miR-1205, hsa-miR-6824-3p and hsa-miR-4719 target C16L gene at VARV genome and F12L gene at VACV gene. This figure is drawn based on the gene’s position of both VARV and VACV, so lengths of genes (kilo base pairs) are not to scale. The same colors between VARV and VACV viral genomes indicate that those genes are genetically similar. VARV F and N genes (combined) exposed significant similarity with the VACV D gene whereas VARV A and J genes (combined) have shown significant similarity with the VACV A gene. Although, all 3 miRNAs showed ~90% identity with both VARV and VACV viral genomic motifs, most importantly, they showed perfect identity at the seed region. Both genes encoded EEV maturation protein where they presented 98% amino acid sequence identity with each other. The EEV maturation protein facilitates the transport of intracellular viral particles to the cell membrane and absence of EEV maturation protein has the inverse effect to infect new cell membrane as well as viral replication.