Gene Target Assay Primer/Probe Sequences (5´-3´) Concentration AE011190.1.a pXO1-MGB PCR F: CATTAAAGTTTTGGCCTGTATAGTCAA 9×10-7mol/L BX_A0201   R: GGATTTGCAGAAGGAATGGAAA 3×10-7mol/L Position: 169299- 170756   Pr: [FAM] CTGCCACCCTTCG [MGB]b 2×10-7mol/L AE011191.1 pXO2-MGB PCR F: CGCTGGCGCTTCAATTCT 9×10-7mol/L BX_B0102   R: AGAGATGACAAAGCAAGGGATGA 9×10-7mol/L Position: 84945- 85382   Pr: [FAM] CCTGCTTTCACTGCTT [MGB] 2×10-7mol/L AE016879.1 Ba chr-MGB F: CCCCATTCAATGTAGCGTTCTAA 3×10-7mol/L BA_5351   R: CTGTTTTATGTACACAAAGATTCGGAA A 9×10-7mol/L Position: 4847165- 4847410   Pr: [FAM] TGGCAATCCCC [MGB] 2×10-7mol/L aGenBank Accession number, locus tag, and position within sequence bReporter dyes to each probe are listed in parentheses. FAM=fluorescein; MGB=minor groove binder

Table 2: Nucleotide sequences of PCR assays developed in this study.