Mouse model and time post infection Estimated bacterial load (cfu·ml-1) PCR pXO1-MGB BA chr MGB BA1 model 24hr, M1 5.83×104 33.17a, b 29.41   24hr, M2 4.00×101 -b -b Dose per mouse (IP route): 24hr, M3 1.45×102 -b -b   24hr, M4 5.50×101 -b -b   24hr, M5 1.05×102 -b -b   48hr, M1 1.83×105 36.24b 32.43 1.72×106cfu 48hr, M2 1.00×102 -b -b   48hr, M3 2.87×104 NT NT   48hr, M4 1.00×102 -b -b   48hr, M5 1.00×102 -b -b   72hr, M1 ndc -b -b   72hr, M2 3.33×100 -b -b   72hr, M3 3.67×103 35.79 34.34   72hr, M4 2.87×103 34.78 34.82   72hr, M5 3.73×104 30.91 30.39   72hr, M6 nd - -   72hr, M7 5.70×103 32.64 31.9   72hr, M8 nd - NT BA2 model 24hr, M1 1.40×102 36.95 -   24hr, M2 1.65×102 36.59 37.58   24hr, M3 4.10×102 34.41 38.86   24hr, M4 3.25×102 37.03 40.54   24hr, M5 1.80×102 36.79 37.79   48hr, M1 5.67×102 31.18 30.69   48hr, M2 1.33×102 34.58 34.72   48hr, M3 1.57×103 30.34 31.04   48hr, M4 1.00×102 -b -b   48hr, M5 3.33×101 -b -b   72hr, M1 7.05×102 -b -b   72hr, M2 4.13×104 28.13 30.05   72hr, M3 4.75×102 35.08 36.02 BA3 model 24hr, M1 1.33×103 34.03 33.55   24hr, M2 1.45×102 -b -b Dose per mouse (IP route): 24hr, M3 6.00×101 -b -b   24hr, M4 nd -b -b   24hr, M5 2.00×102 -b -b   48hr, M1 3.33×102 -b -b 5.45×105cfu 48hr, M2 1.47×104 NT NT   48hr, M3 6.67×102 -b -b   48hr, M4 4.33×103 38.13 34.31   48hr, M5 1.00×102 -b -b   72hr, M1 1.15×102 -b -b   72hr, M2 1.07×104 39.82 39.88   72hr, M3 nd 35.06 39.68   72hr, M4 4.67×103 34.06 32.22 aCT value: PCR cycle number at which fluorescence first detected. (-)=negative PCR; (NT)=not tested. bDNA extract diluted 1:10 to reduce level of PCR inhibitors c(nd)=No bacterial colonies observed

Table 4: Blood culture, PCR, and ELISA data generated from blood samples taken from a replicated murine infection model.