| Sample ID |
Culture (24-48 hr) |
RV-PCR (9 hr) |
| Average CFU/Sample |
Source of Culture Result* |
PCR of Culture (Ct)** |
Culture Result (Pos/Neg) |
Average ΔCt |
Std Dev ΔCt |
RV-PCR Results (Pos/Neg) |
Notes |
| 1185 |
0 |
EC-PE |
25.2 |
Pos |
7.1 |
1.8 |
Pos |
See Footnote (1) |
| 1198 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1199 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1243 |
3.0E1 |
Plates |
15.7 |
Pos |
6.5 |
0.9 |
Pos |
|
| 1246 |
3.0E1 |
Plates |
13.7 |
Pos |
6.5 |
0.9 |
Pos |
|
| 1255 |
0 |
EC |
25.6 |
Pos |
5.6 |
0.3 |
Neg |
RV-PCR Ave. ΔCt from 1:20 dilution |
| 1257 |
3.0E1 |
Plates |
14.6 |
Pos |
6.1 |
1.3 |
Pos |
|
| 1259 |
0 |
EC |
21.9 |
Pos |
0.0 |
0.0 |
Neg |
Heat Lysis Ave. ΔCt = 3.7 |
| 1261 |
0 |
EC-PE |
25.8 |
Pos |
7.8 |
1.2 |
Pos |
See Footnote (1) |
| 1290 |
0 |
EC-PE |
23.8 |
Pos |
8.6 |
0.6 |
Pos |
See Footnote (1) |
| 1291 |
0 |
EC |
25.6 |
Pos |
10.9 |
0.1 |
Pos |
See Footnote (2) |
| 1292 |
0 |
EC |
25.5 |
Pos |
12.9 |
0.2 |
Pos |
See Footnote (2) |
| 1298 |
1.3E3 |
Plates, FF |
12.9 |
Pos |
8.3 |
0.7 |
Pos |
|
| 1300 |
3.0E1 |
Plates |
13.6 |
Pos |
6.7 |
0.8 |
Pos |
|
| 1301 |
0 |
EC |
25.4 |
Pos |
4.2 |
2.0 |
Pos |
Ave. ΔCt from 1:20 dilution; Heat Lysis Ave. ΔCt = 8.4 |
| 1302 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1303 |
0 |
EC |
35.0 |
Pos |
9.6 |
2.2 |
Pos |
|
| 1304 |
0 |
EC |
23.8 |
Pos |
14.8 |
0.1 |
Pos |
See Footnote (2) |
| 1305 |
5.3E1 |
Plates, FF |
13.3 |
Pos |
20.2 |
1.8 |
Pos |
|
| 1306 |
1.8E1 |
FF, RS |
14.5 |
Pos |
16.0 |
1.7 |
Pos |
|
| 1475 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1476 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
2.9E5 |
Plates |
14.0 |
Pos |
23.6 |
0.1 |
Pos |
|
| 1307 |
0 |
EC |
35.0 |
Pos |
6.7 |
0.4 |
Pos |
|
| 1308 |
8.9E1 |
Plates, FF |
13.6 |
Pos |
7.9 |
0.6 |
Pos |
|
| 1309 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1310 |
0 |
EC |
16.2 |
Pos |
8.7 |
0.3 |
Pos |
|
| 1311 |
0 |
N/A |
Undetermined |
Neg |
6.4 |
0.3 |
Pos |
RV-PCR Ave. ΔCt from 2 replicates (1:20 dilution) |
| 1312 |
0 |
EC |
26.0 |
Pos |
7.7 |
0.3 |
Pos |
|
| 1374 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1375 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1377 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1379 |
0 |
EC-PE |
24.8 |
Pos |
12.3 |
0.5 |
Pos |
See Footnote (3) |
| 1382 |
0 |
EC-PE |
27.0 |
Pos |
12.6 |
0.1 |
Pos |
See Footnote (3) |
| 1383 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
4.7E5 |
Plates |
14.0 |
Pos |
24.9 |
0.1 |
Pos |
|
| 1163 |
0 |
EC |
Undetermined |
Neg |
0.0 |
0.0 |
Neg |
|
| 1164 |
0 |
RS |
15.0 |
Pos |
5.9 |
0.6 |
Pos |
See Footnote (4): Heat Lysis Ave. ΔCt = 8.3 |
| 1165 |
5.9E1 |
Plates |
14.7 |
Pos |
8.8 |
0.2 |
Pos |
|
| 1168 |
0 |
RS |
14.9 |
Pos |
6.1 |
0.4 |
Pos |
|
| 1171 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1177 |
0 |
RS |
15.2 |
Pos |
8.6 |
0.7 |
Pos |
|
| 1179 |
0 |
RS |
14.7 |
Pos |
4.9 |
1.0 |
Pos |
See Footnote (4): Heat Lysis Ave. ΔCt 8.7 |
| 1180 |
0 |
FF, RS |
34.6 |
Pos |
9.3 |
0.3 |
Pos |
|
| 1181 |
0 |
EC |
Undetermined |
Neg |
0.0 |
0.0 |
Neg |
|
| 1182 |
3.0E1 |
Plates, FF |
14.4 |
Pos |
7.0 |
0.8 |
Pos |
|
| 1186 |
0 |
EC |
19.9 |
Pos |
7.5 |
0.8 |
Pos |
|
| 1210 |
3.6E1 |
FF, EC |
14.1 |
Pos |
6.9 |
2.0 |
Pos |
|
| 1464 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1465 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1466 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
3.4E5 |
Plates |
13.5 |
Pos |
20.6 |
0.1 |
Pos |
|
*Positive (pos) or negative (neg) culture determination based upon the serial dilution (plates), filter funnel (FF) plate, and/or enrichment culture (EC), re-streak (RS) from
enrichment culture or Promega-extracted concentrated EC (EC-PE). Positive culture results obtained if > 0 Bg colonies were present on serial dilution or filter membrane
plates, and/or Bg-positive PCR results were obtained (Ct < 35) from colonies and/or the EC or RS.
N/A = not applicable; No CFU for analysis and EC not turbid and/or no
growth from RS.
**Cycle threshold (Ct) values obtained from real-time PCR analysis of selected colonies and/or enrichment culture (EC). Positive RV-PCR result based upon average ΔCt≥
6 and T9 Ct ≤ 39.
Footnote (1): EC concentrated 10- to 20-fold and DNA prepared using Promega extraction prior to PCR analysis to confirm culture result (EC-PE).
Footnote (2): PCR repeated to confirm RV-PCR result; 1:20 dilution result shown.
Footnote (3): RV-PCR results based on repeated PCR analysis with 1:20 dilution (1:10 dilution showed PCR inhibition); EC concentrated 10- to 20-fold and DNA prepared
using Promega extraction prior to PCR to confirm culture result (EC-PE).
Footnote (4): Low spore level, post-decontamination sample; sample positive by heat lysis RV-PCR.
Abbreviations: PC, positive control; NC, negative control; TB, true blank. |
