| Sample ID |
Culture (24-48 hr) |
RV-PCR (9 hr) |
| Average CFU/Sample |
Source of Culture Result* |
PCR of Culture (Ct)** |
Culture Result (Pos/Neg) |
Average ΔCt |
Std Dev ΔCt |
RV-PCR Results (Pos/Neg) |
Notes |
| 1260 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1314 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1315 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1316 |
1.8E1 |
FF |
16.8 |
Pos |
9.0 |
0.3 |
Pos |
See Footnote (1) |
| 1317 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1318 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1319 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1320 |
0 |
EC-PE |
27.4 |
Pos |
6.9 |
0.2 |
Pos |
See Footnote (2) |
| 1322 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1324 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1325 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1328 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1329 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1332 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1337 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1479 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
1.8E1 |
Plates, FF |
15.3 |
Pos |
0.0 |
0.0 |
Neg |
Cross-contamination for culture portion only |
| PC |
7.1E1 |
Plates, FF |
15.1 |
Pos |
11.4 |
0.3 |
Pos |
|
| 1341 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1342 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1344 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1346 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1348 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1349 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1352 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1354 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1355 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1356 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1357 |
0 |
EC-PE |
25.0 |
Pos |
7.5 |
0.3 |
Pos |
See Footnote (1) |
| 1358 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1359 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1360 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1361 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1480 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
8.9E1 |
Plates, FF |
15.1 |
Pos |
15.9 |
0.5 |
Pos |
|
| 1238 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1313 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1321 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1323 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1326 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1327 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1331 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1333 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1334 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1335 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1338 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1339 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1340 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1343 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1350 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1473 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
8.9E1 |
Plates, FF |
15.6 |
Pos |
10.1 |
0.1 |
Pos |
|
| 1353 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1362 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
*Positive (pos) or negative (neg) culture determination based upon the serial dilution (plates), filter funnel (FF) plate, and/or enrichment culture (EC).
Positive culture results obtained if > 0 Bg colonies were present on serial dilution or filter membrane plates, and/or Bg-positive PCR results were obtained (Ct < 35) from
colonies and/or the EC or RS.
N/A = not applicable; No CFU for analysis and EC not turbid and/or no growth from RS.
**Cycle threshold (Ct) values obtained from real-time PCR analysis of selected colonies and/or enrichment culture (EC).
Positive RV-PCR result based upon average ΔCt≥ 6 and T9 Ct ≤ 39.
Footnote (1): Culture-PCR result based on repeated analysis and 1:10 dilution.
Footnote (2): EC concentrated 10- to 20-fold and DNA prepared using Promega extraction prior to PCR, to confirm culture results (EC-PE).
Abbreviations: PC, positive control; NC, negative control; TB, true blank. |
